Regulatory

Part:BBa_K082017:Design

Designed by: Jian Zhan , Xunyao Wu   Group: iGEM08_USTC   (2008-10-28)

general recombine system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 35
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 36
    Illegal PstI site found at 50
    Illegal NotI site found at 43
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 36
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 36
    Illegal PstI site found at 50
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to utilize the recombination system repetitively, we design an adaptor where we can put in any part that needs recombining. To accomplish this goal, the restriction sites of SpeI and PstI are placed between lox66 and lox71.In this way, any fragment digested by XbaI and PstI can be inserted between the two recombination sites.

Source

none

References